|
KMID : 1177720120060010009
|
|
Journal of Alternatives to Animal Experiments
2012 Volume.6 No. 1 p.9 ~ p.14
|
|
|
Trends of development of alternative developmental toxicity tests methods using human embryonic stem cells
|
|
Kim Young-Won
Min Jeong-Ran
Jeong Jin-Hee
Jeong Sang-Hee
|
|
|
Abstract
|
|
|
|
Developmental toxicity is one of very important chemical toxicities, which is induced by the exposure of chemicals during embryonic or fetal stages and results in fatal defects of morphology or functions of the offspring. Until now, developmental toxicity test methods are based on laboratory animals or in vitrocultured animal-derived cells or organs. They are relatively expensive and take long time for test and do not sufficiently reflect the real impacts on humans. Therefore, in vitro models based on human embryonic stem cells (hPSCs) have become introduced as an alternative method for developmental toxicity test. We investigated and summarized the key points of the alternative developmental toxicity test methods using human embryonic stem cells. Molecular markers representing differentiation of specific organs or development of critical functions and optimal time for the test of differentiation are suggested. With respect to embryotoxicology, control compounds that mirror strong, weak or none teratogenicity were surveyed to be used in developmental toxicity test to get more accuracy of estimation for real human teratogens. The use of human embryonic stem cells can be regarded as one of future challenges for more precise and efficient models to predict human teratogens.
|
|
|
KEYWORD
|
|
|
Developmental toxicity, Human embryonic stem cell, Alternative method, Differentiation markers
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|